toptestmotor.com
toptestmotor.com Enero 25, 2020


Bacterial DNA stores movie in scientific breakthrough

15 Julio 2017, 12:55 | Verda Sainz

Bacterial DNA stores movie in scientific breakthrough

An image of a hand was stored in living bacteria and then after multiple generations the image on the right was recovered by sequencing bacterial genomes

In a report published today in Nature, Harvard researchers demonstrate that it is possible to archive images and movies in the DNA of living E. coli cells.

The aim is to be able to record data in the genomes of living cells that can be accessed later, providing insights that could help fight disease and deepen our understanding of biology.

To "read" the information back, the researchers sequenced the bacterial DNA and used custom computer code to unscramble the genetic information, which spits out the images. For example, several methods of gene therapy may leave active Cas9 in patient cells, which clinicians would want to turn off. "It's absolutely critical to have an on-switch for gene-editing, but it's just as important to have an off switch", he adds.

While realizing this new concept of molecular recording, Shipman together with second-author Jeff Nivala, a research fellow in genetics at HMS, identified a valuable set of requirements in their analysis that make spacer sequences likely to be more easily acquired and defined sequence features that prevent their acquisition into growing CRISPR arrays-the do's and don'ts of spacer design.

Ultimately, researchers want to place these engineered cells inside living organism where they can automatically record events (or changes) and then remove the smart cells and decode the DNA-stored information for full, sequential play back.

The scientists then similarly translated five frames from the racing horse in motion photo sequence into the DNA. Imagine what progress neuroscience could see, for instance, if scientists could record all the molecular events that a neuron undergoes during the course of its lifetime.

Shipman and his team's choice of a GIF as DNA storage test media is not surprising when you look at their long-term goal of biological recording. Even though the bugs had grown and divided over the week, they had retained the synthetic strands of DNA which Shipman used to reconstruct the images with 90% accuracy. It would be, said Church, analogous to the black boxes carried by airplanes whose data is used in the event of a crash.

Due to its dynamic, if brief, nature, encoding the historic horse GIF presented unique challenges something like sticking an old static image into living bacteria. Then, they introduced their custom sequences into a population of E. coli bacteria, creating temporary pores in the cells' membranes with electric pulses.

The feat is the latest breakthrough in the effort to computerize biomolecular resources.

CRISPR-blocking proteins work by targeting a spot on the CRISPR-Cas9 molecule and binding with it, rendering the molecule unable to cut DNA, the team said.

In a first, researchers converted a movie into a DNA sequence and inserted it into bacteria. This series of photographs, which show a galloping horse, is sometimes cited as being the first silent film. George Church and Jeffrey Macklis to transfer the encoded DNA into the bacteria. For this they turned to the cutting-edge, NIH-funded gene editing technology CRISPR. And this legendary, Nobel Prize-worthy tool basically acts like a computer's copy/paste function - allowing scientists to manipulate DNA in all sorts of ways. The team decided to hack this system for their own purposes, says study co-author Seth Shipman, a neuroscience researcher at Harvard University. They hope to someday use the technology to record cell behavior.

It probably the smallest movies ever made.

A team of scientists achieved a new breakthrough in the process of storing and then replaying data on DNA.



Otras noticias

Tendencias Ahora

Trump hints USA could rejoin Paris climate change agreement
Trump, while addressing a press conference along with Macron, defended his son and said there was nothing unusual in that meeting. Sitting at a table with the children, she introduced herself, "Je m'appelle Melania", or, in English, "My name is Melania".

FSU, Atlantic Division rivals take center stage at ACC Kickoff
However, the conference only has one in the last four years and no one besides Alabama has won since 2010. Rather, it was delivered by John Swofford in his annual Commissioner's Forum address.

Summer heat is on the way
The reason for the showers and storms will be primarily due to a cold front moving into our area...crossing later tonight . Thursday and Friday will be unsettled as the stalled front to our north will finally push southward toward the coast.

Jerusalem shooting: Attack near Old City leaves three dead, police says
Abbas has repeatedly called for non-violent resistance to Israel's occupation without specifically condemning Palestinian attacks. Three Palestinian citizens allegedly fired on police units near the Lions' Gate, a popular religious site in the area.

Wimbledon: Muguruza powers to final
Her most recent came in 2008, when she defeated her younger sister, Serena, for the title. The Brit had her title dream snatched from her in two bruising semi-final sets yesterday.

Security tightened before Bastille Day parade
Shortly after arriving in France on Friday, President Donald Trump commented on the physical appearance of France's first lady. He had also added that the United States was walking out of the accord as it was putting USA industry at risk.

Sister Serena is helping me, insists record-seeking Venus Williams — Wimbledon
No one has beaten both Serena and Venus in a major final. "But she didn't give me much chance to do anything", she said. All of us players have experienced that throughout our careers. "But I would back Jo". "Some kind of plank", she said.

Liu Xiaobo's death a warning to the Vatican
The head of the Nobel committee placed Liu's prize on an empty chair to symbolize his absence from the award ceremony that year. German Chancellor Angela Merkel said Liu Xiaobo was a "courageous fighter for civil rights and freedom of opinion".

Tennis Star Andy Murray Reminds Reporter That Women Exist Too
She last won the title in 2008, but reached the semifinals last year and the Australian Open final this year. Wimbledon is the lone Grand Slam tournament that schedules all 16 remaining singles matches on the same day.

Watch Lonzo Ball Drop 36 While Not Wearing His BBB Shoes
Ball lofted one assist from beyond half court over a defender's head as the defender was busy chasing Kyle Kuzma . Ball had 36 points, 11 assists and eight rebounds in the Lakers Summer League win over the Sixers.